Source: Recombinant gene engineering production, E. coli expression.
一、Product introduction
Long chain LR3 insulin-like growth factor (LR3 IGF-1), a member of the insulin gene family, is structurally similar to insulin precursor proteins and has better growth-promoting effects, which can promote the division, migration and differentiation of most cells.
The Long chain LR3 insulin-like growth factor (LONG-R3 IGF-I) is an analogue of human IGF-I containing 83 amino acids. Compared to intact human IGF-I, there are 13 additional amino acid residues at the n-terminal. Of particular importance is that the third amino acid residue is replaced by the natural Glu with Arg (hence the name R3). Compared to natural human IGF-I, LR3 IGF-I greatly reduces its binding ability to human IGF-I binding proteins. The free concentration is higher, so the biological activity is significantly improved. In multinumber in vitro experiments, LR3 IGF-I showed good biological activity in the concentration range of 0.2-20 ng ml.
二、Product characteristics
| Source | Escherichia coli |
| Appearance | White, yellowish powder |
| Specific activity | Detection activity of rat L6 cell line＞1.0*105U/mg;Human cell line assay activity＞6.7*105U/mg |
| Purity (protein electrophoresis) | SDS-PAGE electrophoretic purity of up to 98%, RP-HPLC purity of up to 90% |
| Endotoxin | ＜0.01EU/µg |
| Molecular weight (mass spectrum) | 9112Da |
三、Storage and transport stability
Storage stability: Stored below -15℃, effective for two years.
Transport stability: refrigerated transport, stable activity.
四、Product advantages
No animal origin: Reconstituted production, no exogenous virus pollution, the production process does not use any animal source raw materials.
Stable quality: mass production (10+g grade production and supply), to ensure stable and continuous batch production; There is no difference between batches and the quality is stable.
High purity: higher than life; Endotoxin and host protein residues were less than the limits of biological products.
Freeze-dried powder: Easy to store and transport.
Compliance with regulatory requirements: the production equipment and production environment meet the relevant regulatory requirements, and the production process fully complies with the GMP guiding principles.
Complete quality documents: According to customer needs, relevant regulatory support documents can be provided.
五、Product use and suggestions
When dissolved: It is recommended to dissolve with PBS, and the dissolution concentration does not exceed 1mg/ml.
Recommended concentration for cell culture: 5-100μg/L.
Recommended cell lines: HEK293, CHO cells to produce antibodies and proteins;
Pluripotent stem cells, mesenchymal stem cells, muscle stem cells
六、Related products
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Recombinant basic fibroblast growth factor bFGF