Storage: Store at -20°C or below, protected from light.

Source: Recombinant Human Basic Fibroblast Growth Factor is produced by genetic engineering and expressed by Escherichia coli.

I. Introduction

The recombinant human basic fibroblast growth factor (b-FGF) is a member of the fibroblast growth factor family. It has been evidenced that bFGF can support the stem cell characteristics of undifferentiated human embryonic stem cells, and stimulate the proliferation of the mesoderm-derived cells and neuroectoderm-, ectoderm- and entoderm-derived cells. In vitro, bFGF is a chemotactic and mitogenic agent for endothelial cells, and can also promote nerve differentiation, survival and regeneration. It has been demonstrated that it is critical for regulating embryonic development and differentiation, and may play a role in angiogenesis, tissue repair, embryogenesis and in vivo neuronal function modulation. Recombinant Human Basic Fibroblast Growth Factor has a molecular weight of 17.2 kDa and is a basic protein.

II. Product Characteristics

III. Storage and Transportation Stability

Storage stability: The lyophilized powder of rhbFGF is stable up to 36 months when stored at -20°C, and up to 12 months when stored at 2-8°C; repeated freeze-thaw should be not more than 3 cycles when it is dissolved in water for injection or sterilized ultrapure water and the resulting solution is stored at -80°C.

Transportation stability: The viability of rhbFGF is stable when it is transported in dry ice for thermal insulation.

IV. How to Use

It is recommended that lyophilized bFGF be dissolved in water for injection or sterilized ultrapure water to obtain a concentration not less than 100 μg/mL and not more than 5 mg/mL for further dilution to the working concentration. Repeated freeze-thaw should be avoided as far as possible.

bFGF after reconstitution: stable up to 7-10 days when stored at 4°C. It should be sub-packaged and stored at -80°C for long-term storage.

V. Advantages

Animals-free: It is produced by recombination, without exogenetic viral contamination, and no materials of animal origin are used in the manufacturing process.

Stable quality: batch production (10+ g production and supply), which can ensure steady and continuous batch production; no difference between batches, for which stable quality can be assured.

High purity: high bioactivity; low endotoxin content; residual host protein below the limit requirement for biologics.

Lyophilized powder: easy for storage and transportation.

Regulatory compliance: The manufacturing equipment and environment conform to the related regulatory requirements, and the manufacturing process fully follows NSF ISO 9001:2015 and complies with GMP.

Complete quality documents: Supporting documents for regulatory compliance are available as per customer requirement.

VI. Product Use

Recombinant Human Basic Fibroblast Growth Factor can promote the proliferation of most cells, and can be used as a component added to cell culture media.

Cell lines recommended: pluripotent stem cell, mesenchymal stem cell and muscle stem cell;

Hybridomas used for antibody production

Vero cells, BHK cells, diploid cells and the like used for production of virions, etc.

VII. Related Products

Recombinant Human Epidermal Growth Factor (EGF)

Recombinant Human Transferrin (TF);

Note：Used only for scientific research